Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 2nd International Conference on Antimicrobial and Antibacterial Agents Renaissance Polat Istanbul Hotel, Istanbul, Turkey.

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Scientific Program Day 1
Scientific Program Day 2

Day 1 :

Keynote Forum

Tony Velkov

University of Melbourne, Australia

Keynote: From breast cancer to antimicrobial: Combating extremely-resistant Gram-negative superbugs using novel combinations of polymyxin B with selective estrogen receptor modulators

Time : 9:30 am-10:30

Conference Series Antimicrobial Congress 2019 International Conference Keynote Speaker Tony Velkov photo
Biography:

Tony Velkov is a principal research fellow & NHMRC CDF Level 2 Industry fellow. He is currently pursuing his research as an associate professor in Pharmacology & Therapeutics in the University of Melbourne.

 

 

Abstract:

Novel therapeutic approaches are urgently needed to combat nosocomial infections caused by Extremely Drug Resistant (XDR) superbugs. This study aimed to investigate the synergistic antibacterial activity of polymyxin B in combination with Selective Estrogen Receptor Modulators (SERMs) against problematic Gram-negative pathogens. In vitro synergistic antibacterial activity of polymyxin B and the SERMs Tamoxifen, Raloxifene and Toremifene was assessed using the micro dilution checkerboard and static time-kill assays against a panel of Gram-negative isolates. Polymyxin B and the SERMs were ineffective when used as monotherapy against polymyxin-resistant (MIC≥8 mg/L) Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii. However, when used in combination, clinically relevant concentrations of polymyxin B and SERMs displayed synergistic killing against the polymyxin-resistant P. aeruginosa, K. pneumoniae and A. baumannii isolates as demonstrated by a ≥2-3 log10 decrease in bacterial count (CFU/ml) after 24 hours. The combination of polymyxin B with Toremifene demonstrated very potent antibacterial activity against P. aeruginosa biofilms in an artificial sputum media assay. Moreover, polymyxin B combined with Toremifene synergistically induced cytosolic GFP release, cytoplasmic membrane depolarization, permeabilizing activity in a nitrocefin assay and an increase of cellular reactive oxygen species from P. aeruginosa cells. Additionally, scanning and transmission electron micrographs showed that polymyxin B in combination with Toremifene causes distinctive damage to the outer membrane of P. aeruginosa cells, compared to treatments with each compound per se. In conclusion, the combination of polymyxin B and SERMs illustrated a synergistic activity against XDR Gram-negative pathogens including highly polymyxin-resistant P. aeruginosa isolates and represents a novel combination therapy strategy for the treatment of infections due to problematic XDR Gram-negatives.

 

 

Keynote Forum

Ahmet Katı

University of Health sciences, Turkey

Keynote: Biosynthesis of silver nanoparticles and their versatile antimicrobial properties

Time : 11:00-12:00

Conference Series Antimicrobial Congress 2019 International Conference Keynote Speaker Ahmet Katı photo
Biography:

Ahmet Katı is currently working as an assistant professor in University of Health sciences, Turkey. He was an industrial microbiologist and working as a Research Scientist in Hayat Kimya R&D Center. He is head of Biotechnology research laboratory, coordinating multidisciplinary studies with other internal R&D teams. He is an expert about preservatives which are using household cleaners and cosmetic products. He received a PhD degree in Biotechnology from Yeditepe University, Istanbul. His research area is focusing on industrial enzymes, preservatives, biocidal products and regulations, developing new antimicrobial agents and probiotics.

 

 

Abstract:

  • Human Virology and Infectious Diseases | Viral Oncology | Antibiotics, Antimicrobials and Chemotherapy | Infection and Immunity | Biofuels and Petroleum Microbiology | Biofilms Formation | Antimicrobial Drugs
Location: Renaissance polat Istanbul Hotel
Speaker

Chair

Ahmet Kati

University of Health Sciences, Turkey

Session Introduction

Lina M Elsalem,

Jordan University of Science & Technology, Jordan

Title: Synergistic effects of antimicrobial peptide WLBU2 combined with antibiotics against multidrug-resistant bacteria associated with wound infections
Biography:

Lina M Elsalem has completed her PhD in Pharmacology from Faculty of Life Sciences at University of Bradford, UK. She is currently working as an Assistant Professor in the Department of Pharmacology at Faculty of Medicine, Jordan University of Science and Technology.

 

Abstract:

Microbial resistance against conventional antibiotics is considered as a substantial health and economic burden to any health care system. Accordingly, research in the antimicrobial field has been directed toward the development of new agents such as the engineered cationic amphipathic peptide WLBU2. The aims of this study are: To evaluate the antimicrobial effects of WLBU2 against multi-drug resistant strains of bacteria including Gram-negative Enterobacter cloacae (ESBL+ve) and Gram-positive Methicillin-resistant Staphylococcus aureus (MRSA) and to assess whether synergistic effects can be achieved upon combination treatment of WLBU2 with conventional antibiotics. Clinical isolates of Enterobacter cloacae (ESBL+ve) and MRSA (n=30 for each) were obtained from wound infections in microbiology laboratories of the Jordanian Royal Medical Services. The Minimum Inhibitory Concentration (MIC) for WLBU2 and conventional antibiotics were determined using the microbroth dilution assay, according to CLSI-2017 guidelines. The antibiotics Ceftazidime, Ciprofloxacin and Ceftriaxone were used for Enterobacter cloacae (ESBL+ve), while MRSA was treated with Oxacillin and Erythromycin. The Fractional Inhibitory Concentration (FIC) index was utilized to determine the relationship between antimicrobial agents upon combination of sub-inhibitory concentration of WLBU2 with conventional antibiotics. The MIC for WLBU2 against Enterobacter cloacae (ESBL+ve) and MRSA ranged from (1.5 to 12.5) μg/ml and (0.78125 to 6.25) μg/ml, respectively. Synergistic and additive effects were achieved in Enterobacter cloacae (ESBL+ve) upon combination treatment with Ceftazidime (n=7/30, 17/30), Ciprofloxacin (n=9/30, 17/30) and Ceftriaxone (n=10/30, 17/30) respectively. Regarding MRSA, synergistic and additive effects were obtained with Erythromycin- WLBU2 treatment (n=2/30 and 10/30, respectively), while only additive effects were observed with Oxacillin- WLBU2 combination (n=14/30). Our data reveal that WLBU2 is a potent peptide with antimicrobial activity against Enterobacter cloacae (ESBL+ve) and MRSA. Our findings also support the development of WLBU2 in combination with conventional antibiotics with potential application for treatment of multidrug resistant bacteria associated with wound infections.

 

Samia Hamma-Faradji

Université de Bejaia, Algeria

Title: Study of the antibacterial activity of strains of Lactococcus vis-à-vis strains of antibioticresistant S. aureus isolated from the ben artisanal
Biography:

Samia Hamma-Faradji is a teacher & researcher in the University of Bejaia, Algeria.

Abstract:

Algeria is a country traditionally a consumer of fermented milk products, which until recently was manufactured in rural areas, mainly for self-consumption, by spontaneous fermentation of locally produced raw milk. On the one hand, non-compliance with good hygiene practices during milking, makes these products in several cases causing food poisoning; on the other hand the increased use of antibiotics for the treatment of dairy cows has contributed to the growing emergence of antibiotic-resistant bacterial strains. The development of natural control means with respect to thèse multi résistant Stains is Born as the use of lactic bacteria endowed with antibacterial activity. In this context, isolation of strains of S. aureus is made from 60 samples of artisanal fermented milk type Ben harvested from 20 farms in the wilaya of Bejaia. Phenotypic and biochemical identification was performed. In order to select strains of antibiotic-resistant S. aureus, an antibiotic susceptibility test was performed for cefotaxime and ceftazidime followed by an analysis of resistance phenotypes. 10 strains of Lactococcus were tested against strains of S. aureus resistant via the spot test. In order to determine the probable origin of the antibacterial activity of these strains, a test of the wells is carried out for the supernatants of lactic strains. The results obtained show that 50% of the ben samples are contaminated with S. aureus, in fact, 60 S.aureus strains are identified. The study of the sensitivity of these strains with respect to cefotaxime and ceftazidime made it possible to select 20 strains of S. aureus with a 32.62% strength of resistance. The analysis of the resistance phenotypes revealed the production of CTX-M-15 β-lactamases in 10 strains. The study of the antibacterial activity revealed zones of inhibition which vary between 15 and 35 mm. However, the well test showed that six strains of Lactococcus showed inhibitory zones that exceeded 15mm after pH neutralization of the supernatant. In conclusion., This high rate of resistance seems to reflect the inappropriate use of antibiotics. Nevertheless, the use of lactic bacteria strains of the Lactoccocus genus endowed with antibacterial activity could be an alternative for having artisanal products based on healthy raw milks.

Mounira Kara Ali

Boumardes University, Algeria

Title: Kinetic models and parameters estimation study of bioethanol production from inulin using P. caribbica
Biography:

Mounira Kara Ali has completed his PhD at the age of 30 years from Frères Mentouri Constantine 1 University and postdoctoral studies from the same university. He is the teatcher of biotechnology. He has published more than 20 papers in reputed journals.

Abstract:

Bioethanol serves as liquid fuel or gasoline enhancer in many countries in response to the progressive depletion of the world’s energetic resources. Production of bioethanol from inulin has been a subject of great interest for many years due to the large amount of existing and not completely developed technologies. The production of bioethanol from inulin, by the yeast Pichia caribbica as bioenergy source was investigated. The obtained results show that this strain was produced ethanol in two steps. Thus, it hydrolyzed 40g/l of inulin to 13.69 g/l and 7.4 g/l of fructose, after 24h, under aerobic and anaerobic conditions, respectively. The yields productions after 72h in these conditions were 0,31g of ethanol/g of inulin and 0,15g of ethanol/g of inulin, respectively. The growth kinetics and modeling of ethanol production from inulin by Pichia caribbica (KC977491) were studied in a batch system. Unstructured models were proposed using the logistic equation for growth, the Luedeking- Piret equation for ethanol production and modified Leudeking- Piret model for substrate consumption. Kinetic parameters (X0, μm, m, n, p and q) were determined by nonlinear regression, using Levenberg -Marquart method implemented in a Mathcad program. Since the production of ethanol was associated with P. caribbica cell growth, a good agreement between model predictions and experimental data was obtained. Indeed, significant R2 values of 0.91, 0.96, and 0.95 were observed for biomass, ethanol production and substrate consumption, respectively. Furthermore, analysis of variance (ANOVA) w as also used to validate the proposed models. According to the obtained results, the predicted kinetic values and experimental data agreed well. Finally, it is possible to predict the development of P. caribbica using these models.

Miyanou Rosales-Hurtado

Université de Nîmes, France

Title: Synthesis of new analogs of diaminopimelic acid (DAP) and lysine
Biography:

Miyanou Rosales-Hurtado has completed her Master’s degree in Molecular and Macromolecular Chemistry at University of Bordeaux, France and Internship course on G-quadruplexes at European Institute of Chemistry and Biology. She is currently pursuing her PhD at the University of Nimes, France.

Abstract:

Infections caused by multidrug-resistant bacteria represent one of the biggest challenges in the medical field. There is an urgent need to develop new antibacterial targets and antibacterial agents to combat multidrugresistance bacteria. For several years, the main target has been focused on the peptidoglycan biosynthesis pathway which provides a potential route to design novel antibiotic compounds. In fact, most bacteria require either lysine, or its biosynthetic precursor, Diaminopimelate Acid (DAP), as a component of the peptidoglycan layer of the cell wall. Nevertheless, DAP/Lysine is not present in mammals, thus, inhibitors of this pathway could provide potential antibacterial agents displaying low mammalian toxicity. In order to fight antibacterial resistance, our work focused on the design and synthesis of new analogs of diaminopimelic acid and lysine.

Melike Tepe

Gebze Technical University, Turkey

Title: Antibacterial effects of deoxsyhikonin produced by hairy root culture
Biography:

Melike Tepe completed a master in the Medical Microbiology Department at Istanbul University, now she is a Ph.D. candidate in Molecular Biology and Genetics at Gebze Technical University. During her PhD studies, she got a scholarship from the Higher Education Institution in the field of Sustainable Agriculture. She has published 1 paper, 2 poster, 1 oral presentation. Her main goal is to produce plant secondary metabolites that have anticancer and antibacterial effects and characterize them by applying several methods, namely FT-IR, HPLC, UV, NMR. She was worked in many science and nature festival which are funded by TUBÄ°TAK.

Abstract:

In nature, plants produce primary metabolites such as proline, glycose, etc. in order to improve its survival while under abiotic and biotic stress conditions, the plant produces secondary metabolites which are main classified of terpene, alkaloid and phenolic compound for adaptation in the environment. In Boraginaceae family, Echium italicum, Alkanna tinctoria, Lithospermum erytrorhizon plants produce phenolic compounds such as shikonin from hairy roots. Among them, Shikonin, which has antibacterial, anticancer and woundhealing activity, is still used in cosmetic and pharmaceutic industry in China and Japan. Hence, aim of this study concerns the investigation of antimicrobial effect of deoxyshikonin which was produced by hairy root culture under abiotic stress conditions. For antibacterial analysis, DMSO (dimethyl sulfoxide) was used as negative and chloramphenicol (C) utilized as positive control. According to our results, deoxyshikonin at 20 mg/ml showed in vitro antibacterial effect against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 15442.

  • Clinical Microbiology | Bacterial and Viral Vaccines | Medical Virology, Clinical and Diagnostic Virology | Diagnosis and Treatment of Infectious Diseases | Bacterial and Viral Vaccines | Vector Borne and Zoonotic Infectious Diseases
Location: Renaissance polat Istanbul Hotel
Speaker

Chair

Hsiu-Jung Lo

National Health Research Institutes, Taiwan

Session Introduction

Zohra Benfodda

Université De Nîmes, France

Title: Rational design, synthesis and antimicrobial properties of thiophene derivatives that inhibit bacterial histidine kinases
Biography:

Zohra Benfodda has completed her PhD in Organic Chemistry at Montpellier University. She is currently working as an Associate Professor at Nîmes University, France.

Abstract:

The emergence of multi-drug-resistant bacteria emphasizes the urgent need for novel antibacterial compounds targeting unique cellular processes. Two-component signal Transduction Systems (TCSs) are commonly used by bacteria to couple environmental stimuli to adaptive responses, are absent in mammals and are embedded in various pathogenic pathways. To attenuate these signaling pathways, we aimed to target the TCS signal transducer Histidine Kinase (HK) by focusing on their highly conserved Adenosine Triphosphate (ATP) binding domain. We used a structure-based drug design strategy that begins from an inhibitor-bound crystal structure and includes a significant number of structurally simplifying intuitive modifications to arrive at the simple achiral, biaryl target structures. Thus, ligands were designed, leading to a series of thiophene derivatives. These compounds were synthesized and evaluated in vitro against bacterial HKs. We identified eight compounds with significant inhibitory activities against these proteins, two of which exhibited broad-spectrum antimicrobial activity. The compounds were also evaluated as adjuvants for the treatment of resistant bacteria. One compound was found to restore the sensitivity of these bacteria to the respective antibiotics.

Negar Zarif Zargarian Talasaz

Dr Masih Daneshvari Hospital, Iran

Title: Defining the most common pathogens in patients with plural empyema who where hospitalized in Dr. Masih Daneshvari Hospital from April 2017 to April 2018
Biography:

Negar Zarif Zargarian Talasaz is a research student in Dr. Masih Daneshvari Hospital, Iran

Abstract:

Objective: To define the most common pathogens in patients with pleural empyema and find the specific antibiotics in order to treat patients more effectively.
Method: In this prospective analytic study all patients who admitted in Dr. Masih Daneshvari Hospital with the confirmed diagnosis of pleural empyema from April 2017 to 2018 were enrolled to our study. Sample of pleural fluid was sent to laboratory for Gram staining and aerobic and also anaerobic culture.
Results: 48 patients with pleural empyema including 40 men and 8 women with the mean age of 43.29 years (range of 5 to77) were studied. Cultures were positive in 6 patients (12.5%); a total of 4 microorganisms were isolated. Aerobic Gram-negative organisms were the most common bacteria in positive culture effusions (66.66%). In this study there were only 6 patients with positive cultures (12.5%), Two patients had Pseudomonas aeruginosa, Acinetobacter baumannii was cultured in two patients, Enterococcus sp., in one
patient and Streptococcus non group was cultured in one patient. Nine patients had positive Gram stain but there was not any growth in their cultures (all of them had rare Gram-positive cocci and two of them also had rare Gram-negative bacilli). The rest of the patients (33/48-68.75%) had negative Gram stain and cultures.
Conclusion: The number of patients who had positive culture was less than expected. We think that there might be a problem in sample collecting, sample transferring, culture providing or the improper usage of broad spectrum antibiotics before referring to hospital and taking the samples.