Antimicrobial and Antibacterial Agents

Bioethanol serves as liquid fuel or gasoline enhancer in many countries in response to the progressive depletion of the world’s energetic resources. Production of bioethanol from inulin has been a subject of great interest for many years due to the large amount of existing and not completely developed technologies. The production of bioethanol from inulin, by the yeast Pichia caribbica as bioenergy source was investigated. The obtained results show that this strain was produced ethanol in two steps. Thus, it hydrolyzed 40g/l of inulin to 13.69 g/l and 7.4 g/l of fructose, after 24h, under aerobic and anaerobic conditions, respectively. The yields productions after 72h in these conditions were 0,31g of ethanol/g of inulin and 0,15g of ethanol/g of inulin, respectively. The growth kinetics and modeling of ethanol production from inulin by Pichia caribbica (KC977491) were studied in a batch system. Unstructured models were proposed using the logistic equation for growth, the Luedeking- Piret equation for ethanol production and modified Leudeking- Piret model for substrate consumption. Kinetic parameters (X0, μm, m, n, p and q) were determined by nonlinear regression, using Levenberg -Marquart method implemented in a Mathcad program. Since the production of ethanol was associated with P. caribbica cell growth, a good agreement between model predictions and experimental data was obtained. Indeed, significant R2 values of 0.91, 0.96, and 0.95 were observed for biomass, ethanol production and substrate consumption, respectively. Furthermore, analysis of variance (ANOVA) w as also used to validate the proposed models. According to the obtained results, the predicted kinetic values and experimental data agreed well. Finally, it is possible to predict the development of P. caribbica using these models.